Chutiya Ram Essay Example for Free

Chutiya Ram Essay Tour Dutt was born on March 4, 1856 in Bengal and she died on August 30, 1877, in the prime of her youth, at 21. She is often called the Keats of the Indo-English literature for more than one reason her meteoric rise on and disappearance from the literary firmament, as also for the quality of her poetry. James Darmesteter pays a befitting tribute to her, The daughter of Bengal, so admirable and so strangely gifted, Hindu by race and tradition, and an English woman by education, a French woman at heart, a poet in English, prose writer in French, who at the age of 18 made India acquainted with the poets of French herself, who blended in herself three souls and three traditions, died at the age of 21 in the full bloom of her talent and on the eve of the awakening of her genius, presents in the history of literature a phenomenon without parallel. Literary Achievements Toru Dutt’s literary achievements lay more in her poetic works than in her prose writings. Her poetry is meagre, consisting of A Sheaf Gleaned in French Fields and Ancient Ballads and Legends of Hindustan. But she compels attention as KRS Iyengar puts it. Her poetry has sensitive descriptions, lyricism and vigour. Her only work to be published during her lifetime was A Sheaf, an unassuming volume in its overall get-up. The Examiner in its August 1876 issue published the review of her book. Edmund Gosse, the then reviewer expressed his surprise To find Miss Toru Dutt translating, in every case into the measure of the original, no less than 166 poems, some of them no less intricate in form than perplexing in matter. He calls it an amazing feat and a truly brilliant success. A review in the Friend of India says. †¦ the versification is generally good, and the translations, we believe, intelligent and faithful. In selecting poems for translation Toru focused attention on the Romantics of French literature, although she also included Chenier, Courier, Lamartine and a few others of the transition period as well as Brizeux, Moreau, Dupont and Valmore who were not Romantics. In France, the Romantic school was born towards the close of the 18th century and in the beginning of the19th, as in England. They asserted the free-play of imagination, simple and direct diction and freedom from any restrictions. The poems that she translated were probably those which could touch the cord of her imaginations and sentiments patriotism, loneliness, dejection, frustrations, illusions, exile and captivity. One remarkable thing about her translation is that she has been able to capture the spirit of the original. No wonder, then, that Edmund Gosse, in his review says, If modern French literature were entirely lost, it might not be found impossible to reconstruct a great number of poems from its Indian version. Not that she has blindly translated. In fact, she has changed words and phrases of the original and substituted them by more appropriate ones without any hesitation which make her work exact and yet free. The verses maintain the rhythm of the original. Though European by education and training, Toru was essentially an Indian at heart. From her childhood her mother had imbued in her love for the old legends from the Puranas, the Mahabharata and the Ramayana. Her readings of the old Sanskrit classics gave her first-hand knowledge of the charming stories. Her woman’s imagination wove myriad coloured picture and she embarked upon her work, Ancient Ballads and Legends of Hindustan, which appeared in 1882, with Introductory notes by Sir Edmund Gosse. Critics have hailed Ancient Ballads as the best work in English. It shows her keen interest in the Indian translations. According to Lotika Basu, a literary critic, Ancient Ballads, for the first time reveals to the West the soul of India through the medium of English poetry. In fact, scholars are profuse in their praise of this work for its finely-knit verses full of vigour and variety. The stories included are of Savitri, Lakshman, Prahlad, Sindhu and others. Toru wrote two novels Bianca and Le Journal de Mademoiselle d’Arvers. The former, an incomplete romance, is in English and the latter in diary form, is the story of Marguerite and is in French. The manuscripts of these works were discovered after her death amid her papers. Both these works have simple plots which sustain the story element, the language is poetic and the characters are clearly drawn. Toru was proud of India’s cultural heritage, her flok-lores, myths and legends, and its rich classical literature. Though English by education, she was an Indian through and through. E. J. Thompson wrote about her, Toru Dutt remains one of the most astonishing woman that ever lived †¦. Fiery and unconquerable of soul. These poems are sufficient to place Toru Dutt in the small class of women who have written English verse that can stand.

Global Warming is False Essay -- Environment Climate Change

A Flawed Opinion Whenever someone thinks of CO2, they think of global warming. They are reminded of Al Gore and his stance on global warming and they automatically think it is true. He has a lot of evidence to back up his theory about how global warming exists and that it is the reason animals are going extinct and also why the climate is changing. Well these facts are wrong and there is evidence to prove it. Global warming is not real and the Earth might even be in a cooling period. There so many reasons to blame people for the epidemic of this false global warming. CO2, a naturally produced gas absorbed by oceans and trees could not be the reason, so what could? Well, nothing can, none of man’s efforts to control the climate will ever succeed against the power of Earth’s natural system of warming and cooling itself. Three great reasons to discredit the theory of global warming are the reason CO2 is not a greenhouse gas, the natural climate change of the earth throughout the ages, and liberal based media’s false facts and propaganda. The great thing about CO2 is that it is not a produced greenhouse gas. John Coleman states plainly in his article to the San Diego Chamber of Commerce about the real story about CO2: â€Å"Here is the deal about CO2, carbon dioxide. It is a natural component of our atmosphere. It has been there since time began. It is absorbed and emitted by oceans. It is used by every living plant to trigger photosynthesis. And we humans, we create it. Every time we breathe out, we emit carbon dioxide into the atmosphere. It is not a pollutant. It is not smog. It is a naturally occurring invisible gas† (3). In this quote he distinguishes the difference between smog and CO2. Smog, a blend of both smoke and fog is... ...problems on fraudulent matters and it is up to the intelligent of the Earth to tell them they are wrong. Works Cited Appenzeller, Tim. â€Å"Signs From Earth.† National Geographic. 4 Feb. 2009. http://ngm.nationalgeographic.com/ngm/0409/feature1/. "Snopes.com: John Coleman on Global Warming." Snopes.com: Urban Legends Reference Pages. 20 June 2008. 4 Feb. 2009. . â€Å"Global Warming: Not So Fast." World Climate Report. 12 Feb. 2009 . Haley, James. Global Warming. New York: Greenhaven P, Incorporated, 2001. Nizza, Mike. â€Å"Failing to Deliver a Jolt on Global Warming.† 21 Apr 2008. 4 Feb. 2009.

Research design and methodology Essay

Despite the fact that the complete genome of the organism was already sequenced, the specific genes coding for the needed enzymes to form pores in the host cell were still unidentified. With this lack of information, this study is formulated and designed. Culturing of B. bacteriovorus HD100 on prey dependent and prey independent set-ups: Predatory (HD) cultures of B. bacteriovorus HD100 will be grown on E. coli in Ca2_-HEPES buffer at 30Â °C, with shaking at 200 rpm (8). Escherichia coli ML35 and E. coli W7-M5 (10) will be used as the prey throughout the experiments. Escherichia coli ML35 will be cultured in nutrient broth (Difco Laboratories), and E. coli W7-M5, a lysine and DAP auxotroph, will be cultured in nutrient broth supplemented with 0. 2 mM lysine and 0. 1 mM DAP at 37Â °C with shaking at 200 rpm. Prey-independent HI strains will be plated on rich peptone-yeast extract (PY) medium (8). Synchronous cultures: Synchronous cultures will be used for performing various experiments as described below. Briefly, fresh bdellovibrios will be added to prey cells in HM buffer (3 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES)-1 mM CaCl. LQ. One mM of MgCl2 will be adjusted to pH 7. 6 using NaOH (10). The organisms will be grown until a final concentration of 1010 bdellovibrios per ml and 5 x 109 E. coli per ml is reached. For proper aeration, volumes will be kept to ? 20% of the flask’s volume and incubated at 30Â °C with shaking at 400 rpm. Synchronous cultures will be examined at intervals for attachment and penetration with a Nikon model L-Ke microscope (Nippon Kogaku Inc. ) equipped with phase-contrast optics and a Nikon model AF camera. Time course Microarray analysis. Time course Microarray analysis will be performed to identify the genes to be expressed during the entry phase, specifically during pore formation on the host cell membrane of B. bacterovorus H100. Microarray slides of B. bacteriovorus H100 will be ordered from Advanced Throughput, Inc Services. Total cellular RNA will be extracted from B. bacteriovorus H100 cells at entry phase using the RNeasy mid kit (Qiagen). The RNA of the organism will also be extracted during the other stages of infection. This will serve as a reference for comparison of the genes expressed and not expressed at the desired stage. Complementary DNA synthesis, fragmentation, labeling, hybridization, staining and washing will be performed according to the Affymetrix B. bacteriovorus H100 GeneChip array expression analysis protocol (Affymetrix). Briefly, cDNA will be synthesized from RNA using Superscript II (Invitrogen) according to the manufacturer’s instructions. RNA will be removed by alkaline treatment and subsequent neutralization. Complementary DNA will be purified with QIAquick PCR purification columns (Qiagen). Purified cDNA will be fragmented by DNase I (Amersham) at 37Â °C for 10 min followed by end labeling with biotinddUTP, using an Enzo BioArray terminal labeling kit (Affymetrix), at 37Â °C for 60 min. Hybridization will be performed in an Affymetrix GeneChip hybridization Oven 640. Washing and staining will be performed using an Affymetrix Fluidics Station 400. Arrays will be scanned with an Agilent GeneArray Scanner G2500A. GeneChip scans will be initially analyzed using the Affymetrix Microarray Suite 5. 1 software, from which PivotData tables will be exported. Raw data from the PivotData Tables will be analyzed in GeneSpring software version 6 (Silicon Genetics), using the parameters suggested by Silicon Genetics for analysis of Affymetrix Microarrays. Real-time PCR: Real-time PCR using the Applied Biosystems 7500 Real-time PCR system will be performed to confirm microarray results. RNA will be extracted from B. bacteriovorus H100 at initial phases of predatory life cycle up to entry phase as described above. RNA will be reverse transcribed into cDNA and simultaneously labelled using the iScript One-step RT-PCR kit with SYBR Green (Biorad). RT-PCR reactions will also be performed to amplify cDNA of housekeeping genes (identified from micro array studies) for normalization of fluorescence values. Identifying the specific hydrolytic enzymes of B. bacteriovorus which are involved in pore formation on host cell membrane. Many experiments showed that B. bacteriovorus H100 releases hydrolytic enzymes during predatory life cycle. According to Thomashow and Ritterberg, glycanases and lipopolysaccharideases are required for pore formation in the prey’s peptidoglycan and LPS layers respectively. The glycanase and/or peptidase could be responsible for weakening the peptidoglycan layer of the prey and thereby responsible for permitting conversion of the substrate cell to a spherical shape (10). Tudor et al. proposed another model for penetration. According to them peptidase is responsible for pore formation but not glycanase (11). Specific enzymes involved in pore formation are not known. The genes identified from the time course micro array technique will be mutated as described previously using suicide vector pSSK10. Resulting mutants will be complemented by using vector pMMB206 (8). Mutants will be analysed for the specific enzymes (using 2D-gel electrophoresis) and their actions on host cell i. e, as a glycanase, LPSase or peptidase will be observed by radio labelling experiments (10). Wild-type B. bacteriovorus H100 and complemented strains will be used as controls. Radio labeling experiments: Escherichia. coli W7-M5, auxotroph for lysine and DAP and cannot metabolize glucosamine, will be radiolabelled as described previously (9,10). Peptide portion of E.coli W7-M5 peptidoglycan will be labelled with [3H] DAP and the lipopolysaccharides and glycan portions of the peptidoglycan will be labeled with [3H]glucosamine. Various mutants and wild-type strains will be tested for predation using this radiolabelled strain. Solubilisation of glucosamine and DAP from labelled prey peptidoglycan will be measured as described previously (11). Briefly, samples taken at intervals will be precipitated with an equal volume of cold 10% trichloroacetic acid for 30 min followed by centrifugation. Resulting supernatants will be assayed for soluble radioactivity in a scintillation counter (Rackbeta II). Two-dimensional gel electrophoresis: The hydrolytic enzymes released by B. bacteriovorus H100 during its predatory life cycle will be analyzed by performing two-dimensional gel electrophoresis. Sample preparation for 2D-gel electrophoresis: Escherichia coli ML35 cells will be challenged with B. bacteriovorus H100 wild-type as well as the mutant strain. Culture fluid will be drawn from synchronous cultures during attachment and entry phases of B. bacteriovorus H100. Culture fluid will be centrifuged to discard any cell debris. Proteins in the supernatant will be precipitated using cold acetone. The precipitated proteins will be separated by centrifugation. The precipitated pellet will be air dried and will be dissolved in rehydration solution (8M urea, 2% CHAPS {3-[3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate}, 18 mM DTT, 0. 5% IPG buffer pH range 4-7; Amersham Biosciences), plus a trace of bromophenol blue. Sample protein concentrations will be determined using the BCA protein assay (Pierce). Resulting protein pellet will be subjected to 2D-gel electrophoresis.

Summary Of The Joy Of Reading And Writing By Sherman Alexie

The Key to Prosperity: For A Native American Sherman Alexie the author of the essay The Joy of Reading and Writing: Superman and Me was born and raised on a Spokane Indian Reservation. Growing up his family did not have a lot of money, yet today Alexie is known as one of the most prominent Native American writers. Alexie reminisces on his childhood when he first taught himself how to read. In the essay The Joy of Reading and Writing: Superman and Me Sherman Alexie suggests, that for Native Americans reading is the key to education and education is the key to prosperity in life. Alexies father was the reason he began to read which later became his passion. His father loved to read, and even though they did not have a ton of†¦show more content†¦They would make him stay quiet in class because most of them did not like to speak during class with their non-Indian teacher. Even though at home they would talk nonstop about anything. These kids did not grow up to have opportuniti es they could have had because they were not given a proper education. The non-Indian teachers did not push the kids to learn and they did not care about their students education. The kids knew that they were expected to fail with their education, and they grew up knowing it was okay to fail because they were Indian. However, Alexie did not accept that. He knew he could pass and that he was smart, so he challenged himself to learn out of the classroom. Reading became the center of his education; he read late into the night, at recess, during lunch, after class, and whenever he could make time to. As a boy he read everything he could find with words on it including all the books his dad had at home, newspapers, library books, cereal boxes, posters, manuals. Even though he loved books he knew reading saved his education and his entire life. His future was opened up to new opportunities because he was educated. Through personal experience I have learned that an education does truly help you succeed in life. Growing up I have had a good education system and been able to push myself to learn. However, I have met some people who have not been as lucky as me. Around eight years ago, I was twelve yearsShow MoreRelatedLiterary Criticism : The Free Encyclopedia 7351 Words   |  30 Pageschange is extremely important.[4][5] Contents [hide] 1 Origin 2 Plot outline 3 Examples 3.1 Precursors 3.2 17th century 3.3 18th century 3.4 19th century 3.5 20th century 3.6 21st century 4 See also 5 Notes 6 References 7 Bibliography 8 Further reading 9 External links Origin[edit] The term was coined in 1819 by philologist Karl Morgenstern in his university lectures, and later famously reprised by Wilhelm Dilthey, who legitimated it in 1870 and popularized it in 1905.[1] [6] The genre is further